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Dataset de Zenodo · Morozumi, Ryosuke

HS-AFM movies of RuvC–DNA interactions under +Mg²⁺ and −Mg²⁺ conditions

Abrir en Zenodo
Licencia
CC BY 4.0uso comercial permitidoTexto de la licencia
Esta licencia permite el uso comercial. Normalmente hay que citar al autor; consulta la licencia para ver las condiciones exactas.
Formatos
AVI, XLSX
Tamaño
12 GB
Actualizado
23 feb 2026

Descripción

This dataset contains high-speed atomic force microscopy (HS-AFM) movies of Escherichia coli RuvC interacting with plasmid DNA under two experimental conditions, in the presence (+Mg²⁺) and absence (−Mg²⁺) of magnesium ions. The repository includes 35 individual cruciform-binding event movies extracted from full-length HS-AFM recordings (15 events under +Mg²⁺ conditions and 20 events under −Mg²⁺ conditions). In addition, full-length HS-AFM movies containing non-cruciform binding events used for the statistical analyses in Figures 3C and 5C are provided; these recordings include 127 non-cruciform binding events under +Mg²⁺ conditions and 126 under −Mg²⁺ conditions, with multiple binding events potentially occurring within a single movie. The repository further includes the complete raw dwell-time datasets (in seconds) used for the statistical analyses in Figures 3C and 5C, the raw cruciform structural classification counts (rabbit-ear, cross-shaped, and no-cruciform) for Figure 1D, and the raw particle height measurements for Figure S4.

Descripción de Zenodo, abreviada.

Archivos (65)

  • AVIRuvC_Mg+_Movie17_1.0s_per_frame.avi594 MB
  • AVIRuvC_Mg+_Movie09_0.5s_per_frame.avi594 MB
  • AVIRuvC_Mg-_Movie06_0.5s_per_frame.avi132 MB
  • AVIRuvC_Mg+_Movie08_0.5s_per_frame.avi148 MB
  • AVIRuvC_Mg+_Movie04_1.0s_per_frame.avi102 MB
  • AVIRuvC_Mg+_Movie01_0.5s_per_frame.avi287 MB
  • AVIRuvC_Mg+_Movie03_1.0s_per_frame.avi594 MB
  • AVIRuvC_Mg-_Movie05_0.5s_per_frame.avi397 MB
  • AVIRuvC_Mg+_Movie06_0.5s_per_frame.avi594 MB
  • AVIRuvC_cruciform_binding_Mg-_Event10.avi13 MB
  • AVIRuvC_Mg-_Movie10_0.5s_per_frame.avi594 MB
  • AVIRuvC_cruciform_binding_Mg+_Event11.avi16 MB
  • AVIRuvC_Mg-_Movie02_0.5s_per_frame.avi594 MB
  • AVIRuvC_cruciform_binding_Mg+_Event01.avi30 MB
  • AVIRuvC_Mg+_Movie14_0.5s_per_frame.avi594 MB
  • AVIRuvC_Mg-_Movie04_0.5s_per_frame.avi594 MB
  • AVIRuvC_Mg-_Movie07_1.0s_per_frame.avi194 MB
  • AVIRuvC_Mg-_Movie08_0.5s_per_frame.avi594 MB
  • AVIRuvC_Mg-_Movie01_0.5s_per_frame.avi594 MB
  • AVIRuvC_Mg+_Movie05_0.5s_per_frame.avi513 MB
  • AVIRuvC_Mg+_Movie07_0.5s_per_frame.avi493 MB
  • AVIRuvC_cruciform_binding_Mg-_Event08.avi13 MB
  • AVIRuvC_cruciform_binding_Mg+_Event15.avi21 MB
  • AVIRuvC_cruciform_binding_Mg-_Event11.avi16 MB
  • AVIRuvC_cruciform_binding_Mg-_Event16.avi33 MB
  • AVIRuvC_cruciform_binding_Mg+_Event02.avi49 MB
  • AVIRuvC_cruciform_binding_Mg+_Event03.avi14 MB
  • AVIRuvC_cruciform_binding_Mg+_Event05.avi18 MB
  • AVIRuvC_cruciform_binding_Mg+_Event06.avi16 MB
  • AVIRuvC_cruciform_binding_Mg+_Event07.avi47 MB
  • AVIRuvC_cruciform_binding_Mg+_Event09.avi14 MB
  • AVIRuvC_cruciform_binding_Mg-_Event02.avi15 MB
  • AVIRuvC_cruciform_binding_Mg-_Event03.avi13 MB
  • AVIRuvC_cruciform_binding_Mg-_Event05.avi15 MB
  • AVIRuvC_cruciform_binding_Mg-_Event04.avi13 MB
  • AVIRuvC_cruciform_binding_Mg-_Event06.avi14 MB
  • AVIRuvC_cruciform_binding_Mg-_Event09.avi14 MB
  • AVIRuvC_cruciform_binding_Mg-_Event15.avi14 MB
  • AVIRuvC_cruciform_binding_Mg-_Event17.avi17 MB
  • AVIRuvC_cruciform_binding_Mg-_Event18.avi14 MB
  • AVIRuvC_cruciform_binding_Mg-_Event19.avi18 MB
  • AVIRuvC_Mg+_Movie15_0.5s_per_frame.avi172 MB
  • AVIRuvC_Mg+_Movie12_0.5s_per_frame.avi582 MB
  • AVIRuvC_Mg+_Movie11_1.0s_per_frame.avi413 MB
  • AVIRuvC_Mg-_Movie03_0.5s_per_frame.avi594 MB
  • AVIRuvC_Mg+_Movie02_1.0s_per_frame.avi91 MB
  • AVIRuvC_Mg+_Movie13_0.5s_per_frame.avi594 MB
  • AVIRuvC_Mg-_Movie09_0.5s_per_frame.avi278 MB
  • AVIRuvC_cruciform_binding_Mg-_Event07.avi14 MB
  • AVIRuvC_cruciform_binding_Mg-_Event01.avi13 MB
  • AVIRuvC_cruciform_binding_Mg-_Event13.avi14 MB
  • AVIRuvC_Mg+_Movie16_0.5s_per_frame.avi594 MB
  • AVIRuvC_cruciform_binding_Mg+_Event04.avi21 MB
  • AVIRuvC_cruciform_binding_Mg+_Event08.avi75 MB
  • AVIRuvC_cruciform_binding_Mg-_Event14.avi18 MB
  • AVIRuvC_Mg+_Movie10_0.5s_per_frame.avi350 MB
  • AVIRuvC_cruciform_binding_Mg+_Event10.avi20 MB
  • AVIRuvC_cruciform_binding_Mg+_Event12.avi24 MB
  • AVIRuvC_cruciform_binding_Mg+_Event13.avi14 MB
  • AVIRuvC_cruciform_binding_Mg-_Event12.avi15 MB
  • AVIRuvC_cruciform_binding_Mg+_Event14.avi53 MB
  • AVIRuvC_cruciform_binding_Mg-_Event20.avi20 MB
  • XLSXRaw cruciform structural classification counts for Figure 1D.xlsx9,3 kB
  • XLSXRaw dwell-time datasets used for statistical analysis in Figures 3C and 5C.xlsx12 kB
  • XLSXRaw particle height measurements for Figure S4.xlsx9,2 kB

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