Effects of group versus individual housing on ovaries in house mice (Mus musculus)

Opis

These data are from an experiment investigating how group versus individual housing influences ovaries in house mice ( Mus musculus ). The data were collected using methods described below. Animals and Housing Treatment Female Swiss-Webster mice ( Mus musculus ; Taconic Biosciences, Germantown, NY) arrived at our facility after weaning at three weeks. Upon arrival, mice were placed into a five-week housing treatment of either individual housing (n = 9) or housing in groups of three (n = 9). Mice in both treatments were kept in standard laboratory rodent cages (30 cm x 20 cm x 15 cm) in a temperature-controlled room maintained at 20 - 22 °C with 30% humidity and a 12:12 light-dark cycle. Standard laboratory rodent chow and water were provided ad libitum . All procedures were approved by the Institutional Animal Care and Use Committee (IACUC) at Utica University (protocol 2021-001). Tissue Processing and Analysis At the conclusion of the 5-week housing treatment, mice were euthanized by CO 2 inhalation and decapitated (Pack and Slepecky, 1995) . The ovaries were subsequently harvested, blind to treatment group, and fixed in 2% glutaraldehyde, in 0.4 M PO 4 (pH 7.2) for 12h…

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Fajlovi (3)

• ZIPOvary micrographs.zip2,8 GB
• DOCXREAD ME.docx16 kB
• XLSXMeasurement data.xlsx30 kB

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