Data from: Anacapa Toolkit: an environmental DNA toolkit for processing multilocus metabarcode datasets

Description

1. Environmental DNA (eDNA) metabarcoding is a promising method to monitor species and community diversity that is rapid, affordable, and non-invasive. Longstanding needs of the eDNA community are modular informatics tools, comprehensive and customizable reference databases, flexibility across high-throughput sequencing platforms, fast multilocus metabarcode processing, and accurate taxonomic assignment. As bioinformatics tools continue to improve, addressing each of these demands within a single bioinformatics toolkit is becoming a reality. 2. Here we present an open access modular metabarcode sequence toolkit, Anacapa (https://github.com/limey-bean/Anacapa/), that addresses the above needs, allowing users to build comprehensive reference databases and process raw multilocus metabarcode sequence data to accurately characterize communities. A novel aspect of Anacapa is our database builder, Creating Reference libraries Using eXisting tools (CRUX), that generates comprehensive reference databases for specific user-defined metabarcode loci. The Quality Control and Dereplication module sorts and processes multiple metabarcode loci and processe merged, unmerged and unpaired reads…

Description from Zenodo, shortened.

Files (16)

• ZIPV4_18S.zip33 MB
• ZIPR-WITS.zip5 MB
• ZIP16S.zip177 MB
• ZIPR-P-H.zip166 MB
• ZIP12S.zip8.5 MB
• ZIPAnacapa.zip73 MB
• ZIPR-mitofish.zip3.5 MB
• ZIPFITS.zip86 MB
• ZIPV8-9_18S.zip24 MB
• ZIPR-CO-ARBitrator.zip225 MB
• ZIPPITS.zip57 MB
• ZIP18S.zip17 MB
• ZIPCRUX_Creating-Reference-libraries-Using-eXisting-tools.zip3.2 MB
• ZIPR_UNITE.zip13 MB
• ZIPCO1.zip484 MB
• ZIPR-Midori.zip24 MB

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